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The fixative was applied in vain; Charnin wanted the girls to look tough and messy.
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But the image is not set until the fixative is applied.
Cell pellet thoroughly mixed with the cell fixative was applied on the formatted and coated CTC slide.
The first fixative was applied for 10 min, and the second for 25 min.
The fixative was aspirated.
The next day, the fixative was changed twice.
After 4 weeks, the fixative was replaced with 70%% ethanol.
The fixative was removed with four washes of PTw.
The fixative was then removed and the cells scraped into 1 ml of fixative, transferred to a microfuge tube and spun down.
The supernatant was discarded, and the fixative was changed at least twice.
The fixative was removed and the cells were washed twice with PBS.
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