Sentence examples for the fixative methods as from inspiring English sources

However, when not available, we suggest using one of the fixative methods as well as checking autofluorescent levels.

Bultitude et al. [ 30] suggested use of Bouin's fixative method for preservation of nuclear details of the tissue samples obtained ureteroscopically.

Finally, there were no clear differences between the ability of the different MAbs to detect the different morphological types of TDP-43 inclusions (i.e. DN, NCI, NII, GCI and granular cytoplasmic TDP deposits), FTLD-TDP subtypes A-D as classified by MacKenzie et al. [ 24] or clear effects of fixative method or post-mortem interval in this screening survey.

The better preservation of nucleic acids obtained with alternative fixatives should be an ulterior point in favor of formaldehyde substitution as the primary fixative, as well as in the determination of molecular prognostic or predictive factors with in situ hybridization.

Just as the fixative evaporates, one drop of 45% acetic acid was applied to the area of the dropped cell suspension.

Immunofluorescence was performed according to Debnath et al., using 2% paraformaldehyde as the fixative [ 20].

Wash twice in PBS to remove the fixative, cyroprotect and snap-freeze specimens, as described below.

The second Pap test, the ThinPrep Pap test (Hologic, Inc., Bedford, MA) contains 30-60% methanol as the fixative [ 6].

Cook in the same method as above.

After overnight fixation, the fixative was washed out with PBS prior to immunolabelling as for midgut wholemounts.

After removing the fixative, the cells were washed three more times with 50 mmol/L NH4Cl and processed as described in the immunofluorescence section.