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The first three samplings were made at LARSA: 20 fish par family were randomly sampled (n = 600) when yolk-sac resorption was complete (about two months old), 50 fish per family were randomly sampled (n = 1500) at 15 weeks after exogenous feeding began (about 4 months old), and 50 fish per family were randomly sampled (n = 1500) at 2136 degree-days in September 2006 (about 7 months old).
The number of final BASs obtained from the first three samplings yielded 9 BASs in the case of HIV integrase, as will be discussed in the next section.
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VvAP10 transcript levels were high at the first three sampling times after SA treatment, but declined thereafter.
In both the groups, mean values remained relatively constant at the first three sampling times (T0, T1 and V5) and then increased moderately but significantly at VF. Differences between groups were not statistically significant.
The sensitivity analysis on PaCO2 considering the first three sampling times of all seven sheep strengthened this result, given that the differences with the baseline were all highly significant (p = 0.0004 at 60 minutes, p < 0.0001 at 90 minutes, and p = 0.003 at 210 minutes).
Looking at the clustering of columns, which mirrors the distances among the different stages of berry ripening, it is evident that the bunch order reflects the sequential succession of samples, while there is a clear difference between the first two samplings and the following three.
In the first two samplings (days 7 and 14) larvae were compressed between a slide and a cover slip due to their size.
The first two samplings included all calves, and the three latter, calves II and III.
Applying a two-way hierarchical clustering analysis to these variations, a clear difference between the first two samplings (up to 14 days after véraison) and the following three (from 28 to 49 days after véraison) emerged, thus suggesting that the most relevant changes in protein expression occurred in the first weeks of ripening.
The score plots show good delineation between precap and postcap and also give a clear time trajectory for the first five sampling events, where the first samples taken in each state have the majority of the variability explained by PC1.
During the first four sampling weeks, the TOC/TN ratio increased to 4.99.
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