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Using tritium-labeled BPA, the researchers conducted the first saturation binding assay to precisely characterize how strongly BPA binds ERR-γ.
Fortunately, [H]BPA is now commercially available; thus, in the present study we performed the first saturation binding assay for direct exploration of the binding characteristics of BPA.
While many studies depended on naturally occurring mutations, the use of radiation and chemical mutagens dramatically boosted the generation of phenotypic mutants and eventually led to the first saturation screens allowing for the systematic identification of genes involved in specific processes [ 6].
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In the first case, saturation throughput and delay performances are analyzed as a function of distance for a single source-destination pair.
The first initial saturation distribution is suitable for a pair of fluids with large density difference to gain stability, whereas the second distribution is more realistic to simulate the injection processes.
The data presented here constitute the first comprehensive saturation mutagenesis analysis of a metallohydrolase active site and reveal that the strict conservation of the second-shell metal binding residues in eukaryotic arginases does not reflect kinetic optimization of the enzyme during the course of evolution.
The values of the flatband voltage shift measured at the first and second saturation were slightly higher than the values predicted from the Coulomb blockade theory.
The design load was estimated using conventional design criterion based on the saturation of material at the first unloading flight, saturation of the entire upper half of the drum and saturation of the airborne solids.
In the second case, saturation throughput performance is compared for increasing number of source nodes in the ad hoc network.
No new issues emerged after the thirteenth interview (saturation).
After the second session saturation was deemed to have been reached, so no third session was organised.
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