Sentence examples for the first hrm from inspiring English sources

The first HRM used the 48 6-fish genomic DNA pools comprising that 288-fish as template.

This is the first HRM approach to screen the entire coding region of the BRCA1 and BRCA2 genes using one set of reaction conditions in a multi plate 384 well format using specifically designed primers.

In total, 2,772 out of 2,796 (2,592 in first HRM round, and 204 in the second HRM round), or 99.1%, experiments were evaluable for screening of mutations/variants by HRM analysis.

The second HRM used genomic DNA from the six individual fish in the six-fish pool where the mutation was detected.

The first, Methylation-Sensitive HRM (MS-HRM) [ 18, 19], relies on primers designed to amplify the target independent of its methylation status.

When the second, repeated HRM analysis was compared to sequencing results, specificity increased to 94%, as well as the accuracy, 94% (kappa of 0.82); but the false negative rate also increased to 5%.

All mutated samples were subject to a second HRM and DNA sequencing analyses in order to validate the results.

All mutations were found in heterozygosity and were confirmed in a second HRM and DNA sequence analysis.

This is the first extensive validation of HRM on FFPE samples using the detection of EGFR exons 18 to 21 mutations and KRAS exon 2 mutations.

Conclusions: For the first time, we used HRM and MLPA to identify BRCA1 and BRCA2 mutations in Algerian patients with a personal and family history suggestive of genetic predisposition to breast cancer.

This is the first study in which the HRM method has been properly validated as a scanning method for EGFR and KRAS mutations in a large sample set showing accurate mutation detection with 100% sensitivity.