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This manual sorting process was repeated two additional times and the final sorted neurons were transferred to a PCR tube containing extraction buffer XB (47 μl) from PicoPure Kit (KIT0204, Life Technologies, Carlsbad, CA, United States), incubated (42°C, 30 min), and the mixture was stored at −80°C until library preparation.
These keys are then sorted in the shuffle task (sortByKey) and the reducers combine all the outputs of the shuffle task and partition the final sorted results according to the prefix (a, c, g, n, t) of the suffixes.
Now, these keys are sorted in the shuffle task (sortByKey) and reducers combine all the outputs of the shuffle task and partition the final sorted results according to the prefix of the suffixes (lines 12 15).
The parental cell line contained 2 % CD44v3highALDH1high cells but the final sorted cells contained 18 % CD44v3highALDH1high cells, a 9-fold enrichment.
Gating was set to obtain 80% (or greater) enrichment for p16-positive cells, which resulted in ~5% of total cells being selected, for a total of 1-1.5 × 10 cells in the final sorted fraction.
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A 1.7-fold increase in display and fluorescein binding compared to the wild-type intein was observed in this final sorted pool, and the display levels of the 4 4 20-intein 4 4 20-inteinestored to that ofusionunfused 4–4–20 protein.
The m-way-merge algorithm merges the sorted m sub-arrays into the final output sorted array.
At the end, each researcher reread the sorted data and finalized the final theme.
Immunofluorescence analysis of the final cultures showed that sorted TGFP + cells generated predominantly mutually exclusive single T(Bra) positive mesoderm and single Sox2 + neurectoderm.
The final analysis is limited to the sorted peptides, thereby reducing the complexity but keeping all of the characteristics of the proteome.
After obtaining the final score list s, it is sorted in descending order and the top m, m
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com