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The final insert size was 4682 bp in this AAV-LS1L-GFP vector.
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Amplification of the 14 18-kb 14 18-kbge sizeles (with an estimated final inserangeze of 10–12 kb) did not resamples sufficient material (most likely because of unsuccessful adaptor ligation) and with not further included in the process.
In these cases a gap was introduced between the scaffolds so that the final sequence had the size of the average insert size of the BAC library.
Gaps can occur in the fragmented BAC assemblies since the insert size of the pair end reads is 500 bp.
The final distance d k is then computed as a maximum likelihood estimate based on the mean insert size.
The insert size of the library was approximately 200 bp.
The insert size of the library was ~306 bp.
The actual insert size excluding adapters was ~150 bp.
The average insert size of 12 clones was 1.7 kb (based on PCR of inserts).
As the mean insert size of library 25 is relatively large (463 bp) compared with the average nanochromosome size, mapped reads from this library provide a manner to generally validate our final assembly.
The average insert size of the clones with inserts was 122 +/- 44 kb (SD).
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