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The best transitions (3 5 per precursor) were selected by manualinspection of the data in Skyline and scheduled transition lists were createdfor the final assays.
The final assays were performed using Human Aβ (1−40) or Aβ (1−42) colorimetric sandwich ELISA kits (BioSource, Invitrogen) by following the manufacturer's instructions.
All three treatments were then run for a further 25 generations prior to the final assays of fecundity being performed.
SNPs with SNP score >0.4 were included in the final assays, comprising two primer pools (200 SNPs were included on both arrays for quality control purposes).
We continued to use this cross for its less severely reduced GLV production in comparison to homozygous ir LOX2 plants, which likely permitted growth and reproduction comparable to ir PI and WT in 2011 that was essential for plant matching prior to the final assays of Manduca spp. mortality and plant reproduction (M4, Figures 4 and 9).
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The final assay volume was 200 μL.
The separation science plays a key role in pharmaceutical industry ranging from impurity profiling to the final assay for monitoring the finished products.
The final assay was shown to be specific and linear within the analytical range, with an acceptable CV for repeatability and intermediate precision.
Xylose was determined by D-xylose assay kit (Megazyme), specifically designed to measure this sugar in samples containing up to 5 mg of glucose in the final assay reaction without any interference.
The final assay concentrations of the enzyme and substrate were 0.75 nM and 50 nM, respectively.
However, it would be obvious to utilize SPS in the final assay.
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