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The final assay volume was 200 μL.
The separation science plays a key role in pharmaceutical industry ranging from impurity profiling to the final assay for monitoring the finished products.
The final assay was shown to be specific and linear within the analytical range, with an acceptable CV for repeatability and intermediate precision.
Xylose was determined by D-xylose assay kit (Megazyme), specifically designed to measure this sugar in samples containing up to 5 mg of glucose in the final assay reaction without any interference.
The final assay concentrations of the enzyme and substrate were 0.75 nM and 50 nM, respectively.
However, it would be obvious to utilize SPS in the final assay.
Content of the final assay can also be customized to suit diagnostic needs in different geographic areas.
The final assay did not contain sufficient DTT to reduce the disulfide bonds of insulin (data not shown).
Thirdly it is important to determine if the final assay is to be performed at one or many stringencies.
The primer set for the rcbL gene was subsequently removed from the final assay to improve performance.
The final assay concentration of tracer was 1.2+/−0.08 nM and the compound concentrations ranged from 37 pM to 157 µM in a total volume of 88 µl.
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