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The extracts were analyzed using an Agilent 1100 HPLC system.
After degrading the silk dyeings in 100°C oven up to 7 days, dye was extracted from each silk and the extracts were analyzed using the HPLC-DAD-MS analysis.
The extracts were analyzed using UV-Vis, HPLC and FTIR techniques.
The contents of rhein in the extracts were analyzed using the validated HPLC method [ 15].
The extracts were analyzed using a 4000 QTrap (Applied Biosystems/MDS Sciex) mass spectrometer.
The extracts were analyzed using reverse-phase chromatography followed by mass spectrometry.
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Samples from the extract were analyzed using gas chromatography (CP 3900; Varian).
Then, the extract was analyzed using optimized maltodextrin (MD) modified CE method for separation of TOL enantiomers.
The extract was analyzed using GC-MS (Agilent 5973-model, USA) equipped with C-18 column (30 mm tubular column diameter, 0.25-mm internal diameter and 0.25-μm film thickness).
We utilized a simple extraction format, based on consecutive extraction steps, followed by a rational choice of UPLC-MS analysis, according to the lipophilicity of the extracts, wherein the aqueous extracts were analyzed using a HILIC and organic extracts by a RP method.
The leaves extracts were analyzed using the Quadrupole-Time of Flight Liquid Chromatogram/ Mass spectrometer (Q-TOF LC/MS) and five compounds were successfully identified in Aquilaria subintegra.
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