Exact(5)
To mass standardize the total chlorophyll concentrations, this data was multiplied by the extraction volume (1 ml) and divided by the fresh weight of the samples (mg chl/g FW).
The extraction volume of HCl was adjusted proportionately.
After weighing approximately 10 mg for each sample the extraction volume is adjusted accordingly to a ratio of 30 μl extraction solution per mg of AIR.
We obtained the extraction volume by indirect means, as described in schematic form in Figure 2. We denote this procedure as "volume determination using two standards" or VDTS.
Chlorophyll content was calculated using the following formulae: Chlorophyll a mg / g FW = [ { 12.7 × A 663 – 2.69 × A 645 } / 1, 000 × W ] xV Chlorophyll b mg / g FW = [ { 22.9 × A 645 – 4.68 × A 663 } / 1, 000 × W ] xV Total carotenoid content = 1, 000 A 470 – 1.82 chlorophyll a – 85.02 chlorophyll b / 198 Where W is the fresh weight and V is the extraction volume.
Similar(55)
However, the peak areas were much smaller with 133,017, 139,258, and 135,774 AU, because the analytes were diluted to the whole extraction volume plus the void volume of the trap, which remains in the tube after the injection.
The total nicotine quantified on the QCM (ng) was multiplied by the methanol extraction volume, divided by the crystal's exposed surface area of 3.8 cm2 (the exposed diameter reduces from 25 mm to 22 mm due to the 0.15 cm housing 'lip') and by puff number to present total nicotine per area per puff (ng/cm2/puff).
The same observation was made for the detection of AdV at a concentration of 10 copies/ml (panel 1) with the RespiFinderSMART-22 by using the advised extraction volume of 100 μl.
In the study, orthogonal array designs (OAD) were employed for the optimization of the extraction parameters: type of IL, pH of the sample, extraction volume, ultrasonic time and salt concentration.
Neonatal DBSS from the eight participants were retrieved from the DNSB, and DNA was extracted from one or three DBSS disks, 3.2 mm in diameter, using Extract-N-Amp Blood PCR Kit (ENA)(extraction volume: 200 μL) (Sigma-Aldrich) or QIAamp DNA Blood Micro Kit QIAA)(extraction volume: 75 μL) (Qiagen).
The conclusions are that the sample amount, the rinsing time, the extractant volume (extraction variables), and the acid silica gel amount (purification variable) were statistically significant.
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