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The extracted specimens were mounted on microslides in a drop of distilled water.
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Gross examination of the extracted specimen showed a monolocular cystic mass covered with a fibrous capsule-like surface and containing brownish viscid fluid.
This in agreement with the finding that extracted specimens possess crossing points approximately 1.5 cycles lesser than those of their unextracted counterparts.
For prospectively analyzed HSV-2 specimens, the average crossing point for extracted specimens was 14.69, while the average crossing point for the same specimens analyzed in unextracted form was 16.26 (a difference of 1.57).
Using this factor, a comparison of sensitivities of the use of extracted and unextracted specimens in PCR was reconsidered: In the context of this work, using 5 μl of extracted specimen was actually the equivalent of analyzing only 4.8 μl of original specimen.
At present, one of the few methods of combating the effects of the fungus has been to extract specimens from the wild and keep them safe in captivity, away from the sources of infection, as a way of preserving their genes for the future.
Gathered around a display case of excavation tools, Niles explained how he would extract specimens from the ground during the day and then bring them back to his hotel room to pack them for safekeeping in the evening.
The extracted DNA specimens were stored at −20°C until used.
The extracted RNA specimens were subjected to RT-PCR using SuperScript III (Invitrogen, Carlsbad, CA) to make cDNAs.
The quality and validity of the extracted DNA specimen was assessed by inclusion of β-globin gene-specific primers in the PCR reaction; only specimens with detectable β-globin were used in this analysis.
Cells may also adhere to different devices that are utilized in saliva sample collection (e.g., cotton, foam, and hydrocellulose), causing lower quantities of DNA to be present in the extracted saliva specimen.
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CEO of Professional Science Editing for Scientists @ prosciediting.com