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Panel (A) photographs show the extent of mineralization (bar = 10 μm).
The extent of mineralization is an important factor to understand the osteoblast function.
Nanopore diameter caused the transition in the extent of mineralization by the osteoblasts.
Alizarin red S staining was used to study the extent of mineralization in osteoblasts cultured on flat and nanoporous surfaces.
The extent of mineralization of lindane was determined by quantifying the release of inorganic chloride ions and analyzing the percent residual lindane in the medium.
The combined treatment further improved the extent of mineralization and BFR over PTH alone (P < 0.05) at endocortical surfaces and recapitulated the effects of PTH and PRO alone on trabecular surfaces.
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Besides the carbon mineralization, the extent of nitrogen mineralization and the mass balance of nitrogen were conducted for three pH values (pHo 3.0, 6.2 and 11.0).
We also showed that the extent of surface mineralization including the strongly adhered surface minerals positively correlated with the length of the grafted pSBMA brushes (degree of polymerization, DPs, Figure 6c), further supporting that the surface zwitterionic motifs directly participated in the templated surface-mineralization.
a The extent of calcium mineralization on various nanosurfaces over different days calculated from the alizarin red S staining.
Cells were harvested after days 7, 10, and 14, and alizarin red S staining was then performed to analyze the extent of calcium mineralization.
The reduction in WFPS with time may be influencing the extent of C mineralization as the days progressed because reduction in the water-filled pores during the storage might be curtailing the microbial access to the substrate [42, 52].
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