Sentence examples for the extension time from inspiring English sources

MR. HANCOCK -- Well, I don't need the extension time, Your Honor.

The extension time required for every cycle in these PCR procedures is more than 3 min to obtain long PCR products, which is very time consuming.

The periodicities observed around 320 CE, 400 CE, 630 CE, and 820 CE may be related to the change in global climate, since each duration coincides with the extension time of Swiss glaciers.

Additionally, to amplify long flanking sequences, the extension time of the PCR procedure in these methods is usually more than 3 min/cycle, resulting in these methods taking more than 1 day to complete, even for products < 1.5 kb (Liu and Chen 2007).

The extension time, y (min), was set at 1 min per 1 kb.

The reaction conditions may need to be adjusted if other polymerases are used, especially the extension time.

The optimized annealing temperatures and the extension times are provided in Additional file 1.

Distributions at different forces are color-coded as the corresponding extension traces in A. All the extension-time trajectories shown in this work were mean-filtered using a 1 ms time window.

The problem worsened when the chain extension time was shortened or when the amplicon size was relatively long (>500 bp).

Given the finding that EG had relatively low PCR inhibition, we reasoned that it might be possible to reduce the chain extension time from the standard 60 seconds to a shorter time without adversely affecting PCR performance.

Because the polymerase extension time is fixed in the library amplification step, longer fragments may not amplify in the initial PCR stages, but all short fragments will.