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What's the expression we use?
"I hope Madame le Consul will forgive me for the expression we use in English – saying we do something 'half-assed.' With the French, in my experience, it's always 'whole-assed.' Tonight is another example of how things are not done halfway". Everyone laughed.
So what kind of culture did you want to create when you started your company? A. Probably the biggest lesson I learned as we started to grow was — and this is a more sanitized version of the expression we use — "Don't hire jerks, no matter how talented".
It is the medley of humility, grace, and delight of working together in "the bowl" (the expression we use to describe Fishbowl).
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For normalization of the expression we used the 18S RNA TaqMan probe (4319413E, Applied Biosystems).
In the next step, to further investigate the source of variation in the expression, we used multivariate ANOVA.
To further assess the expression, we used in situ hybridization employing an antisense probe of one of the coreceptors.
For normalization of the expression we used the 18S RNA TaqMan probe (4319413E, Applied Biosystems, Forster City, CA) in the respective master mix according to the suppliers suggested conditions (Applied Biosystems).
To obtain the aligned expression, we use the Viterbi algorithm to align the time points of a patient to the most likely states of the HMM.
To investigate adaptive variation in gene expression we use the teleost Fundulus heteroclitus (killifish) [ 23, 29].
For the rhodopsin expression, we used the transgenic line Tg (rho2 EGFP) cu3 zebrafish expressing EGFP as shown previously (35).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com