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The expression trend between miRNAs and their host genes is highly correlated for both delayed implantation and activation, suggesting that host genes and miRNAs are co-transcribed.
In the case of VWA5A, the expression trend is similar in MEFs and MCF7 cells but the difference in expression levels between the WT and MTA1 knockdown in the MCF7 cell line is marginal.
Western blot analysis in panel Fig. 6B confirmed the expression trend of MTA1 after castration at the protein level, with the exception of an undetected increase at postoperative C7+5 as demonstrated in RT-PCR assay.
The expression trend of their precursors was very similar to that of their corresponding miRNAs, except for pre-miR-6, which was significantly up-regulated under activation (Fig. 5C).
Moreover, all of the miRNAs maintained the expression trend.
The expression trend of VviPNLinNer1 was quite different in 2011 GT-produced berries.
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RT-qPCR was used to validate the expression trends of selected genes identified as being differentially expressed by microarray analysis.
Dramatic perturbation is evident in the expression trends of members in all three classes in the ES (GCNF−/−) mutant.
Table 1 summarizes the expression trends observed for probe-sets that are mapped to the 77 miR-seeds displaying the most significant increase of activity in tumors.
Although the absolute expression ratios generated by iTRAQ and immunoblotting methods are not the same, the expression trends reflected by both methods were congruent and confirmed the aberrant expressions of candidate proteins across the MCF10AT model of disease progression.
Table 2 summarizes the expression trends observed for probe-sets that detect transcripts carrying predicted target sites for miRNAs, whose predicted targets were negatively-expressed to them in the most significant manner.
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