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In order to determine if the patterns of expressed genes during fracture healing were reflective of stem cells, we compared the set of genes that were differentially expressed during fracture healing to the expression sets that are associated embryonic stem cell and mesenchymal stem cells.
Adjacency matrix was then calculated using the correlation matrix of the expression sets.
Interestingly, however, the data indicate that PPARα-tr may act rather independently by altering the expression sets of genes different from those regulated by the canonical receptor.
To obtain a profile taking into account the expression sets of both tumour types, significantly regulated genes were identified independently for both groups.
In other words, the low overlap between the MA and NI networks can not be explained by the size of the expression sets (n = 5) used in their construction.
The expression sets were converted into a matrix with the exprs function, then split in two: condition A and condition B. P values were calculated with the Window Welch t test [ 35] (with default parameters) with the pegase function.
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MAL13P1.14 encodes an ATP-dependent DEAD box helicase and interacts with four other members of the expression set, including PFL0815w (a DNA-binding chaperone), and two uncharacterized proteins, PF07_0106 and PFA0410w.
The expression set was downloaded from the ArrayExpress repository in the form of R expression set (http://www.ebi.ac.uk/arrayexpress/files/E-GEOD-16011/E-GEOD-16011.eSet.r).r
The expression set was collapsed to the gene level by taking the maximum probe for each gene.
Gene median centring was performed on the expression set, where the median of the expression values for a specific gene across all samples was subtracted from that gene.
Based on the results above, proteins having long polypeptide stretches (>50 residues) before the first TM or between TM1 and TM2 were excluded from the expression set.
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