Exact(8)
Mean ΔΔCt values ± SE were transformed (2-ΔΔCt) and shown as the expression relative to the control.
The transcript abundance of genes is shown as the expression relative to the wild-type (WT) plant.
At our 0 h time point, the expression relative to ubiquitin of ACS2d, ACS6a, ACS6b, ACS9a, ACS9b and ACS9d was > 0.001.
A gene by gene analysis of the expression relative to the reference sample revealed a high agreement between array data and QRT-PCR data (Table 4).
The individual GSM sample file expression values were transformed into EF values corresponding to the expression relative to the series mean.
Validation of the microarray experiment was performed by measuring the expression relative to GAPDH for a subset of genes, by using qRT-PCR.
The expression levels of each gene from each group of animals were calculated as per cent of the expression relative to the control NTG animals after normalization for β-actin.
Treatment with 100 μM AgNO3 raised the PUN1 mRNA about 3-fold whereas 50 μM HgCl2 and 10 μM CdCl2 doubled the expression relative to control cells (Fig. 1, left panel).
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