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cDNA derived from the reference pool, which we labelled with Cy5, was used as the expression reference.
Furthermore, a P-value was determined by dividing the absolute difference between the expression reference and condition arrays by the sum of the technical and biological variation on both arrays.
They have major biological key role as they represent the highest variations in regards to the expression reference [see Additional file 3]. Figure 4 presents similar examples to these of Figure 3, but this time, only extreme values were used in the analysis.
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For example, Entrez Gene users can save an expression that captures the logic of their complex search, but if the expression references previous searches in the history then the expression is not useful to others.
The expressions reference a wide range of mathematical topics: number theory, geometry, topology, combinatorics, analysis, mathematical physics, applied mathematics, computational complexity and mathematical physics.
Because of the inherent variation in the expression of reference genes the use of multiple reference genes rather than one reference gene is recommended to ensure reliable normalization of real-time PCR [ 10].
Four normal tissue samples, derived from non-neoplastic mammary glands resected during tumor surgery, were included in the study as CXCR4 expression reference.
They use different algorithms to analyse the variation in the expression of reference genes, and that may result in different recommendations for the most suitable reference gene.
Abundances of the 23 unigenes were calculated relative to the expression of reference genes DlFSD1a, EF-1a, and eIF-4a.
Results were normalized against the expression of reference genes ODC and RPL8 using CFX Manager software (BioRad).
In the pig however, very little is known about the expression of reference genes, certainly in combination with real-time RT-PCR.
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CEO of Professional Science Editing for Scientists @ prosciediting.com