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Lentiviral vector are commonly used to alter the expression of targeted genes.
Moreover, artificially designed antisense RNAs have become powerful tools to downregulate the expression of targeted genes.
Onesuch therapy, antisense gene therapy, is a technique based on the ability of single-stranded DNA or RNA sequences to alter the expression of targeted genes.
This has led to the design of a wide variety of modular artificial transcription factors (ATFs) that can stimulate or inhibit the expression of targeted genes.
Short double-stranded RNAs, known as short interfering RNA (siRNA), can be used to specifically down-regulate the expression of targeted genes in a process known as RNA interference (RNAi).
A major challenge in cell-based biotechnology is to engineer gene regulatory systems that can detect signals defined by users and then effect desired changes in the expression of targeted genes.
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The expression of target genes was normalized to the expression of GAPDH.
Inhibition of METTL3 or METTL14 had also been shown to increase the expression of target mRNAs26,49.
This system, derived from genes in Neurospora crassa, uses the transcriptional activator QF to induce the expression of target genes.
PcG proteins form two major Polycomb repressive complexes, PRC1 and PRC2, to silence the expression of target genes.
These findings demonstrate that exogenous plant miRNAs in food can regulate the expression of target genes in mammals.
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