If the center of rotation varies as a function of the sinogram number, implying an imperfect experimental alignment, the projections can be rotated according to the computed angle to compensate for the misalignment.
The conformation in Figure 6e is representative of the extended structure similar to the conformation shown in Figure 6a, which supports the experimental CCS alignment for both 4,4′-MDA and the larger 2,4′-MDA conformation.
Simulators can often learn their parameters from an experimental alignment file.
Concerning the analyses of the experimental data, our alignment filtering consists of (i) discarding alignments consistent with a SNV from intragenomic origins, (ii) retaining the unique alignments, and (iii) safely analyzing the U and MU regions.
This is due to the fact that, in the experimental setup, the alignment of the plane of Phantom 2 has had a tiny deviation from a angle to the incident THz beam. Figure 3 Left to right, in radians: phase of acquired complex phantom image, phase image generated using the circle fitting data and knowledge of phantom geometry, and difference image.
The accuracy of our method is further supported by the experimental controls: the alignment of Abp1-GFP to itself and the separation between GFP-Sla2 and Sla2-GFP.
AL contributed to the experimental designs, sequence alignments, bioinformatics, and with drafting the manuscript.
For correct alignment, the experimental and simulated spectra must be centered at the same chemical shift value, and have equal digital resolutions.
Both methods rely on the experimental determination of the alignment tensor for all domains in the multidomain system.
In the case of a guest dye, the experimental measurement of the alignment obtained from a UV/Vis dichroic ratio gives the order parameter of the associated electronic transition dipole moment (TDM) of the dye, rather than that of the long axis of the dye.
