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During the experiment, solution was heated and cooled using a Peltier element.
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Throughout the experiment, solutions were constantly aerated.
The experiment result shows that this solution performs an almost 50% better than other solutions.
To avoid evaporation of this solution during the experiment, the solution was only added to 12 wells at a time.
Additionally, the microlysimeter design functioned reliably throughout the experiment, achieving solution flow rates analogous to the unconfined plots.
At this point in the experiment, the solution was simply brought up to temperature, refluxing any water vapor to prevent evaporation.
When indicated, animals received tetracycline in the drinking water (2 mg/ml) 4 days before cell injection and throughout the duration of the experiment; the solution was refreshed every second day.
Immediately before the experiment, the solution was re-homogenized.
Just prior the beginning of the experiment, the solution is ultrasonicated.
The control group (Group 1) received orally throughout the experiment a solution of 2.5% (v/v) DMSO/tween 80.
In the experiment, PBS solution was first injected into the microchip and the measured signal was set as the baseline signal.
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