The DNA fragments were analysed by agarose gel electrophoresis, and were found to be of the expected sizes.
In this manner, the expected sizes of different tandem arrays were determined.
The expected sizes of the LR-PCR amplicons were confirmed by gel electrophoresis.
PCR gave bands of the expected sizes ranging from 1.1 to 1.5 Kb.
The expected sizes of PCR products were 302 bp (CEN1), 344 bp (CEN16), and 288 bp (PGK1).
Following agarose gel electrophoresis, PCR fragments of the expected sizes were extracted using a gel extraction kit (Qiagen) and cloned in pGEM-T easy vector (Promega).
The amplified cDNA bands were 400 and 369 bp, which are the expected sizes for MCT1 and MCT4, respectively (Fig. 1A, lanes 2 and 6).
Using all four primer pairs, we obtained PCR products of the expected sizes from R. felis cultivated from the initial isolate [2].
PCR products of the expected sizes were observed in each case with cDNA derived both from platelets and from their precursors, megakaryocytes.
In the pupal samples the predominant bands run at 53.5 kD and 56.3 kD, the expected sizes for isoforms II and III.
We anticipated the possible outcomes of NcoI digestions of genomic DNA with the expected sizes of different loci shown in Figure 3B.
