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In cry single mutants (w1118; ; cryb/01), regardless of light intensity, the evening phase remained near the light-off time point of the previous entrainment period, ZT13.65 (L), ZT13.72 (M), and ZT14.28 (H).
As a consequence, PRR-dependent suppression of CCA1 and LHY during the day results in the derepression of the evening phase genes.
Loss-of-function mutations in SbPRR37 and short day abolished the evening phase expression of SbPRR37 and caused significant effects on flowering time and expression of SbCO and SbFT, the Sorghum orthologs of CO and FT. Variation at both genes, Ppd-H1 and SbPRR37 showed significant effects on expression of the FT orthologs and flowering in the respective inductive day-length.
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Each phase of the game represents a daily activity that occurs at the start of the day (Phase 1), at lunchtime (Phase 2), in the afternoon (Phase 3) and at the end of the night (Phase 4).
The electrojet strength (Fig. 3(a)) in the noon phase of November 8 is either reversed, or very low compared to the control day.
As with lesions in the HCRT-ko mice vlPAG lesions produced a significant increase in the number of REM bouts (F1, 14 = 7.762, P = 0.015) and decrease in the length of wake bouts (F1, 14 = 6.107, P = 0.027) during the night phase (Table-1).
Therefore the night experiments were specifically performed in the second half of the night phase.
During the night phase cultures were bubbled with 10 ml/min air, and (passively) cooled down to 23 26°C.
To this aim, Platynereis larvae were sampled between 45 and 75 hpf (with the night phase between 56 and 64 hpf).
Leptin does not appear to need to be in phase with melatonin to present an effect, as already demonstrated in diurnal animals (ewes), but in the rat, since both hormones peak during the night phase, this could refine the modulatory effect of leptin on melatonin synthesis.
Twelve healthy subjects, after judging the emotional valence of the faces in the evening (learning phase), either slept normally in the subsequent night, with sleep recorded polysomnographically (sleep condition), or remained awake (wake condition) according to a cross-over design.
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