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Furthermore, we required that the fragment-specific depth estimate exceeded 40 or the estimated template input exceeded 200.
Importantly, we showed that this is not the case here as the estimated template copy numbers of the cells in figure 3 F (y-axis) do not correlate with increased average transcription rates (fig. 3 D).
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The lowest estimated template modeling score (TM-Score) was 0.46 ± 0.15 for MtAqp-1 and the highest of 0.74 ± 0.11 for both MtAqp-5 and MtAqp-9 (Table 2).
This is also in line with the fragmentation measurements with the 50 samples with the highest C>T/G>A levels strongly trending towards having lower estimated template numbers in the FTH1 assay.
We estimated templates of transient visual ERPs separately for each stimulation rate from the temporally jittered sequences.
The PCR products were electrophoresed in 2% agarose gels to estimate template concentration.
Therefore we used the contralateral intact acetabulum and femoral head as mirrored template to determine the estimated HJC.
In 0.5 unit of polymerase there are about six cycles left before the ratio of estimated template and estimated polymerase reach unity.
To calculate in Equation (8), we use the following equation, (9) where represents the distance of the two sequence residues at the two aligned positions, is the conditional probability of on and is the conditional probability of the distance on the template estimated from the contexts (denoted and ) of the two sequence residues.
The surface area extent covered by the reservoir was estimated using the grid template method.
To increase robustness of this estimate, we include p pseudo counts each of which contributes n d i l - 1, where n d i l is the template number estimated from the dilution series.
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