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These are listed in the "Essential revisions" section.
Please see our response to the second point of the essential revisions.
The essential revisions that we request are: 1) Please provide data to show that a morinda diet does not affect egg-laying in Drosophila melanogaster.
In the two months suggested for revisions and as they were not part of the essential revisions, we could not also address the Rif1 and Rif2 ChIP requests in the variety of telomerase RNA conditions.
As requested and as described above in the response to the essential revisions, we have now also done Sir4 ChIP, in wild-type, sir2∆, sir3∆, rif1∆ and rif2∆ strains that we constructed, with expected outcomes.
We have performed all of the experiments requested by Reviewer 1, as described above in our response to the essential revisions; we did ChIP for TERT in sir2∆ and sir3∆ strains and Sir4 ChIP as well in sir2∆, sir3∆, rif1∆ and rif2∆ strains.
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Our goal is to provide the essential revision requirements as a single set of instructions, so that you have a clear view of the revisions that are necessary for us to publish your work.
The essential revision requirements are: (1) repeat the chromosome titrations you did for Chr12 in strain YPS1009 using the Chr8 strains derived from NCYC110, and this new analysis must include spike-in controls for RNA-seq; (2) much better annotation of the strains used both in main text and in the supplemental.
Before publication, the authors need to make the following essential revisions: 1) The authors refer to the study of Georgescu et al. (EMBO, 2009) and suggest that the observed clamp-OB CTD interaction provides "an alternative explanation" for the reduced DNA synthesis seen by them.
As Sir4 ChIP was part of the requested essential revisions, which we performed in multiple strains including rif1∆ and rif2∆ as requested here, this request has now been addressed above and the new data are shown in Figure 6.
[Editors' note: the author responses to the re-review follow.] Essential revisions:The consensus reached was this is a valuable study well worth publishing, and it was strongly felt that the data set is an important part of the study and should be made public.
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