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In most cases, measurements were performed using a single-cell time-lapse approach, quantifying the equilibration in one cell over a certain time period.
The equilibration in a given time could be regulated by not only formation and differentiation of CSCs into non-CSCs during symmetric and asymmetric divisions but also de-differentiation of the resulting non-CSCs back to CSCs.
Following the equilibration in KRB for 30 min at 37°C or 4°C, the brain slices were incubated in KRB containing 15 µg/ml F-Aβ40 and 20 µg/ml AF633 labeled transferrin (AF633-Trf) (Invitrogen-Molecular Probes, Carlsbad, CA), a clathrin mediated endocytosis marker, for 30 minutes at 37°C or 4°C.
This is similar to the transition that occurs during the equilibration in the apo simulations).
The fourth and last step was the equilibration in which the initial conditions were set for the next run.
The aortic ring was exposed to Ca2+- free K-H solution for 15 minutes after the equilibration in normal K-H solution.
Similar(53)
Likewise, 0.4 0.5°C decrease in the temperature at which 50% denaturation occurs was observed upon a two-fold increase in the equilibration time in isothermal experiments (Fig. 1C, squares).
In addition, the equilibration process in our isopiestic apparatus possesses a fairly fast rate, which is practical even for the samples of the molality less than 0.05 mol · kg−1.
These pressures are mostly higher than the equilibration pressures in the slow-cooling models, where many planetesimals equilibrate in partially crystallised magma oceans.
Upon the imposition of a ΔpH (pH 7.6in/pH 6.8out), 22Na+ uptake in proteoliposomes containing LmrA-MD was observed above the equilibration level in empty liposomes consistent with sodium-proton co-transport (Figure 3F).
In this new regime, we observe a dramatic departure from the equilibration seen in more disordered devices: edge states with opposite spins propagate without mixing.
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