Plasmid DNA was isolated from the equal quantities of the cells grown overnight (stationary phase) or in 3 hours (logarithmic growth phase) using "GenElute Plasmid Miniprep Kit" (Sigma, USA), treated with EcoRV restrictase and RNAse A and resolved in 1% agarose gel.
Based on the synthetical consideration of the performance improvement and economic cost, 10% silica fume content by the equal quantity substitution of cement is optimal for the compressive and flexural strength of steel-fibre reinforced RAC.
Moreover, the beams with advanced spirals exhibited deformation ductility values 2.10 and 2.60, respectively, demonstrating this way improved post-peak deformation ductility compared to the beams with equal quantity of commonly used stirrups.
Once all the regions come to have the same weight, i.e., the periods of [40s, 60s], and [120s, 140s], we can see all the stations use equal quantity of network bandwidth.
When all the regions come to have the same weight, i.e., the periods of [40s, 60s] and [120s, 140s], we can see all the regions use equal quantity of network bandwidth.
MeJA (200 μM) was then added to the cultivation media; an equal quantity of ethanol was used as the control.
Mix the crème de cassis with an equal quantity of the poaching syrup.
To our surprise, 9 (75%) of the 12 NSCLC cell lines expressed only or predominantly DNMT3B and ΔDNMT3B forms lacking exons 21, 22 or both 21 and 22 whereas the HBE cells expressed equal quantity of DNMT3B and ΔDNMT3B transcripts with and without the exons 21 22 (Fig. 1B).
As with the other two sABs, when we polymerized actin in the presence of an equal quantity of sAB-4 no filaments were found in TIRF.
Compared to beams with equal quantity of the commonly used stirrups the measured increase of the torsional strength for the tested beams under imposed twist that locks the spirals was 18%, 16% and 14% for the beams with transverse reinforcement spacing at 200 mm, 150 mm and 100 mm, respectively.
