Your English writing platform
Discover LudwigExact(11)
Fluorescence intensity and analytical sensitivity were determined by first diluting one volume of Eu standard with nine volumes of the enhancement solution to be tested, and then the obtained solution were serially diluted with the enhancement solution.
In the enhancement solution of this technology, lanthanide ions rapidly dissociate from the DTTA chelates due low pH (3.2).
After incubation and washes, the enhancement solution was added and specific hybridisation signal detected by measuring the emitted light.
The enhancement solution and diethylenetriaminetetra acetate (DTTA) chelates used in the DELFIA technology meet these criteria to a high degree.
and the enhancement solution itself is stable upon storage at +4 °C for at least 18 months (Table 3).
Standard dilutions of Sm, Tb and Dy were prepared and tested as described for Eu in the enhancement solution with the most intensive Eu fluorescence.
Similar(49)
The concentrations of the components of the enhancement solutions were primarily optimised with respect to fluorescence intensity of Eu, fluorescence signal stability, dissociation kinetics of EuDTPA chelates and Eu β-diketone 3 Eu β-diketone 3on velocomplex
The velocity of fluorescent complex formation and the stability of the fluorescence signal in the enhancement solutions were determined by adding 5 μL of EuDTPA-labelled AFP antibody and 195-μL enhancement solution into microtitration plate wells that were shaken for 5 min at room temperature and measuring the fluorescence as a function of time.
In the developed enhancement solution the relationship between the Ln concentration and the fluorescent signal is linear up to 10 nmol/L of La and the fluorometer is linear up to 5 × 10 fluorescence counts with the instrument settings shown in Table 1.
The fluorescence from the cups is measured after 100 μL of the developed enhancement solution was dispensed and the cups incubated for 5 min at +35 °C.
The fluorescence intensities of Eu and Sm in the developed enhancement solution as well as the achieved analytical sensitivities compare well with the established 2-NTA-based enhancement solutions.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com