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Water molecules were added at the end of refinement using the automated method provided in PHENIX.
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Towards the end of the refinement, TLS refinement as implemented in phenix.refine was used [53].
At the end of the refinement the R factor and Rfree were 0.162 and 0.180, respectively.
At the end of the refinement (consisting of one loop in this work), the final attacked image J is obtained.
For HI-6sarinnonaged-mAChE residues 490 498 were omitted at the end of the refinement.
Towards the end of the refinement, water molecules were evident in the electron density maps and were included in the model.
Therefore, regular tryptophan in double conformation was inserted at the very end of refinement.
At the end of the SHELXPRO refinement the R factor and Rfree (all data) were 0.108 and 0.146, respectively.
At the end of the beta testing and refinement phase, the guides will be ready for field testing at additional sites.
At the time of this study, the SimplyRNA HT system was at the end of beta testing, and future refinements to the kit may increase yield quantity, quality and precision.
If the precision is the priority order, it is necessary to do a further refinement in the end of the program on these reconstructed 3D points.
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