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The new system which we describe eliminates the need for additional screening of positive clones, improves the efficiency of transposition, and reduces the cost and time required for high throughput baculovirus production.
(A ) The efficiency of transposition depends on the ratio of transposase to DNA.
Chief among these is an apparent decrease in the efficiency of transposition with increasing cargo load, particularly for transposons greater than 6 kB [42], [48], [49].
In the future, it would be good to compare the efficiency of transposition of the new element with sleeping beauty or some previously isolated Tc1-like element, although these Tc1-like elements such as SB and FP were seldom used for fish transgenesis.
The efficiency of the reaction in vivo is unlikely to be so high and this is dealt with in Figure 3G and the section of text entitled 'The efficiency of transposition in vivo.' Many transposons, and mariner in particular, have a wide host range.
Similar(55)
The efficiency of Himar1 transposition is a function of transposon size, with a 38% decrease for every 1-kb increase in transposon size [ 23].
Given the high efficiency of transposition, the number of new insertions identified in on-going remobilisation efforts (~ 10 per week) exceeds our current capacity to keep, expand and cryopreserve all of them.
These multiple pathways are similar to the multiple layers of a "mille feuilles" (Rigal and Matthieu 2011), and their accumulation ensures the efficiency of the control of transposition.
These may also lead to improvement of the efficiencies of mariner transposition systems as genome manipulation tools.
Consequently, provided the cost to the host is < 0.5, and with high efficiency of transposition, the element will spread.
Owing to the efficiency of this in vivo transposition system, we have recovered, identified and characterized a large number of insertions that provide a direct view of the regulatory activities associated with specific genomic regions.
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