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Transgenic (i.e., elt-2::mTomato::HIS2B expressing) embryos were collected at different times to analyze the efficiency and timing of the gland cell death as assessed by YFP expression.
The structure of eukaryotic chromatin and chromosomes influences accessibility of proteins and multi-protein machines to eukaryotic DNA, thereby influencing the efficiency and timing of DNA metabolic processes such as transcription, replication, DNA repair and recombination.
Several factors are taken into account to judge if a modification of the protocol brings about an improvement for reprogramming: (a) the efficiency and timing of colony appearance (b) the number and type (oncogene or not) of factors used, which might depend on the delivery method, the somatic cell type and the co-use of chemicals and (c) the absence of permanent genomic manipulation.
A clear understanding of factors that influence the efficiency and timing of initiation is lacking, although histone modification, nucleosome positioning, and transcription have been implicated [ 5- 11].
The absence of a direct role of Hat1p in the efficiency and timing of these origins could be explained by the possibility that Hat1p/Hat2p does not interact with ORC at these origins and that the S-phase recruitment of Hat1p is solely mediated by Hat1p/Hat2p-Hif1p.
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However, the remaining uncertainty on aspects such as resuscitation efficiency and timing of intervention just might gather enough rumble for a thirteenth round.
Exploring time horizons: What role do efficiencies and timing of effects play? 3.
2. Exploring time horizons: What role do efficiencies and timing of effects play?
In contrast, the incorporation of fluoride ions decreased the solubility of the DF-Ap layer, thereby decreasing the efficiency and delaying the timing of gene transfer on the layer dose-dependently.
Furthermore, although inter-clonal variation was seen in differentiation efficiency, there was no overall difference in the efficiency or timing of neural and cardiac differentiation between FRDA and control iPSCs, suggesting that a reduced amount of frataxin does not affect the initial commitment to neuronal or cardiac fate.
Breakdown voltage, afterpulsing, detection efficiency and timing jitter of the irradiated APDs were characterized and shown to be in the range acceptable for quantum communications.
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