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Here too, we are following a strategy that uses reconstituted systems to monitor function, in correlation with high-throughput mutagenesis to survey the effects of sequence variation.
Spo0F and the N-terminal receiver domain of NtrC (NT-NtrC) have similar topologies but low sequence identity, enabling a test of the effects of sequence on folding.
A procedure was used that was derived from variations of the Corsi test, designed to test the effects of sequence structure and movement path length in humans.
The effects of sequence distribution of two types of poly(propylene-ethylene) (PP-PE) copolymers, with propylene being the dominant component, on the associated crystallization behavior have been analyzed.
By quantifying and rationalizing the contribution of key motifs to transmembrane helix association, our work offers a route to direct the design of novel sequences for use in biotechnology or synthetic biology (e.g. molecular switches) and to predict the effects of sequence modification in known transmembrane domains (for control of cellular processes).
In addition, these results suggest caution in the interpretation of data solely obtained with minigene constructs to study the effects of sequence variation on splicing.
Similar(23)
This filter was implemented to separate causal from bystander mutations and to further diminish the effects of sequencing errors.
The latter observation, however, is consistent with the effects of sequencing error: errors are concentrated towards the ends of reads [5], [8], [28].
We implemented strict criteria to reduce the effects of sequencing errors.
In this way only reads with relatively high quality were used, thereby reducing the effects of sequencing errors.
Using the rhesus macaque as an example, we document the effects of sequencing errors, gaps in sequence and misassemblies on one automated gene model pipeline, Gnomon.
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