To ensure a direct role for PI3-kinase in destabilisation of ER α, we determined the effect of overexpression of constitutively active PI3-kinase on ER α protein levels in MCF-7 cells.
On the contrary, co-treatment of quercetin together with MG-132 did not revert the effect of MG-132 mono-treatment, suggesting that quercetin may directly or indirectly interfere with proteasome-dependent degradation of Mcl-1 in addition to the already observed destabilisation of the corresponding mRNA.
Even though miRNAs repress the protein output of their target genes as a combined effect of mRNA destabilisation and translational repression, a recent study has reported transcript destabilization to be the main contribution to miRNA target deregulation [ 46].
Bonincontra et al [18] and Calandrini et al [19] reported that although the general effect of IPA treatment was destabilisation of the macromolecular structure, the collagen conformation had changed, resulting in alteration of the collagen molecular dimensions.
This data illustrates that the destabilisation effect of imperfect peptides upon a helix is dependent upon the location of the imperfection, and that if helicity is the goal, removal of helix-disrupting imperfections by gel filtration may be more effective than direct purification of an unfolded peptide.
Similarly, Arafat often used the threat of destabilisation and assassination to get Arab regimes to fund the Palestinian cause.
"The PM said that the tragedy had brought into sharp focus the consequences of destabilisation in eastern Ukraine.
As the mode of destabilisation can be quite varied the scope to exploit substrate variants as reversible inhibitors is substantial.
The votes for Brexit and Donald Trump demonstrated the threat of destabilisation of old political and economic orders if too many are left behind.
The apoptotic effect of α-TOS involves lysosomal and mitochondrial destabilisation as well as caspase-3 activation (Alleva et al, 2001; Neuzil et al, 2001b).
