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Table 3 lists the slope estimates for individual chemicals and the dosing protocols, as well as the threshold estimates for both protocols.
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Since most of the animals in the control group died even before receiving the planned endotoxin dose (according to the dosing protocol), the mean total endotoxin dose was approximately one third (252 ± 128 μg/kg) of the full dose received by the ADVOS group (764 μg/kg).
The dosing protocol used was identical to the Fornai studies.
At the EC50 dose for induction, half of the mice were sacrificed while the remaining half continued in the dosing protocol, until all mice had lost their righting reflex.
The dosing protocol was well tolerated.
Details of the dosing protocol were reported elsewhere [ 24].
The mixture dose response studies demonstrated dose-related decreases in activity regardless of the dosing protocol.
We used two experimental designs for the dose response that differed only in the dosing protocol used to administer all the chemicals.
Data for two nonmutagenic chemicals (DDT and dieldrin) were eliminated from the analysis because of the complexity of the dosing protocol used.
The incremental concentrations of methacholine chloride prepared from the dosing protocol were 0.0625, 0.25, 1, 4, 16, 25, and 50 mg/mL.
Mixture stock and dosing solutions were prepared daily by codissolving pyrethroids in corn oil (Sigma Chemical Co., St . Louis MO, USA) according to the dosing protocol described below.
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