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The pyrosequencing analysis revealed that different H. pylori strains dominated the three samples; the dominant sequence of one of the samples had a single bp substitution relative to the others'.
A 745-bp NIb-CP fragment of PPV genome, containing the hypervariable region encoding the CP N-terminal end was amplified by RT-PCR from each sample and directly sequenced to determine the dominant sequence.
Conversely, in the presence of an ectopic nodule, T2-WI can be used to score this zone, as this is the dominant sequence for the TZ.
Nevertheless, T2-WI has been assumed to be the dominant sequence for the identification of PC in the TZ when interpreting imaging findings of a mpMRI exam [42, 43].
However, as there are no established guidelines that suggest scoring a TZ lesion that protrudes into the PZ using the dominant sequence from the TZ (rather than PZ), we deem that in this scenario the experience of the radiologist and the knowledge of prostate anatomy and specific morphological features of BPH (e.g., regular capsule and margins) are more important than ever.
None of the contaminants picked up in these NTC experiments included the dominant sequence of the hair shaft experiments.
Similar(34)
We determined the single nucleotide polymorphism and deletion-insertion polymorphism alleles of the dominant sequences from HLA-DQA2 to DAXX for these CEHs.
The dominant sequences are those that result in a methane explosion and occur with a frequency of 6.5 × 10−8 year−1, while the other sequences are much less frequent.
We then found, for each protein, the number of sequences that met this criterion of being dominant in any season, the average duration of occurrence for the dominant sequences of each protein (measured in number of seasons), and the degree of dominance (calculated by summing the number of samples that were dominant sequences and dividing by the total number of samples in each season).
Compared to pyrosequencing, the dominant sequencing technology in metagenomics, our composite read strategy already provides a >30-fold increase in sequencing capacity per dollar at similar or higher accuracy (Table 2).
Based on phylogenies (Supplemental Figures S3-S4) and the prevalence of the dominant sequences (Figure 2), the rapid expansion of influenza during the epidemic peak consisted largely of the clonal expansion of one clade.
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