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The Himalayan motion of QTP uplift of ca. 20 Ma most likely drove the diversification between Central Asia and North America.
This measure uses the assumption that, although diversification rates were not constant, at any point in time the diversification between two clades occurred at the same rate.
To identify bacteria responsible for the diversification between the two root-associated microhabitats we employed a linear model analysis (Supplemental Experimental Procedures) to determine bacterial OTUs significantly enriched in root and rhizosphere compared to unplanted soil.
At the sequence level, the majority of the diversification between Dichaete and SoxN can be found in regions outside of the HMG domain, which could drive interactions with specific binding partners, and in each gene's regulatory regions, which determine where they are uniquely or co-expressed [ 45].
Our study provides insights for the detailed process, by which genes for DPOR have been eliminated from the chloroplast genome, and further analysis will shed light on the diversification between the nuclear and chloroplast gene expression networks during the evolution of land plants.
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This study also highlights the importance of the North and South American route in the global diversification between the Northern and the Southern Hemisphere [ 95].
Overall, we observed a strong coherence between the single gene and the concatenated tree topologies regarding the diversification pattern between the microclusters.
Our approach focusing on the sex-specific effects pattern was also justified by the clear diversification between genders in the distribution of many confounding factors included in the analyses.
In contrast, the diversification patterns between strains of the same microcluster were highly variable.
The diversification age between Central Asia and QTP of ca. 20.32 Ma implies a response to QTP uplift and extension as a geological event.
This suggested that the intron insertion mechanism newly appeared at least at the time of diversification between the eukaryotic and prokaryotic ancestors, or that the introns in the prokaryotic and eukaryotic common ancestor, the progenote, genomes disappeared in the prokaryotes, as suggested previously [ 23].
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