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We cloned a putative A. cellulolyticus creA gene that is highly similar to the creA genes derived from other filamentous fungi, and isolated a creA disruptant strain by using the disruption method.
Several processes of reference analyses such as the disruption method, mannan hydrolysis, and glycogen hydrolysis were selected and optimized in order to get precise and reliable results.
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Although like many bacteria (Hughes et al. 1971; Chisti and Moo-Young 1986; Benov and Al-Ibraheem 2002; Donsì et al. 2009), P. putida cells are routinely disrupted in the laboratory using French-press, no detailed analysis of this disruption method has been published.
This approach involves film formation of the viologen using the micellar disruption method followed by transformation of the film into the nano/microstructures using a potential sweep technique.
The difference in the outcome can hardly be due to the barrier disruption method, as only a slight variation exists in the application method (differences in cotton swabs and cotton balls).
The biogas potential was found to depend strongly on the species and on the cell disruption method applied.
Of the two different disruption methods compared, the enzymatic treatment approach was superior to the physical disruption method.
After incubation, 1 mL of the supernatant was collected, and DNA was extracted using the physical disruption method described above.
These photovoltaic efficiencies of the films prepared by the electric micelle disruption method were higher than those of the films prepared by the common coating method.
In addition, the results obtained by this method were compared with the results obtained by the Zirmil-beating cell disruption method and the QIAamp Fast DNA Stool Mini Kit.
Ultra Turrax was the best cell disruption method, and refrigeration was the best preservation method.
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