In addition, our case highlights the difficulties of laboratory detection of h-VISA-phenotype, particularly when it is unstable and arises from a fully susceptible VSSA isolate (vancomycin MIC ≤1 μg/ml) under treatment with vancomycin.
Clearly it is not, nor do the team know why their absence prolongs the period that the disease is latent.That is an important lesson about the difficulty of extrapolating laboratory results into the real world.
Graham replied to Findlay regretting that "although we have been engaged in this sort of investigation for the past few years, we do not think that, at present, much help has been given to the clinician"—but the reason he gives for this ("mainly because of the difficulty of the laboratory methods involved") indicates that they are beginning to shift away from Findlay's position.
This work demonstrates the difficulty of relating adiabatic laboratory experiments to the spontaneous heating and ignition of piles of stored coal.
The major drawbacks of the visual MVD counting method are its inherent subjectivity and the difficulty of standardisation between laboratories.
At this level, the book is a fascinating account of the difficulties of designing laboratory experiments to dissect unambiguously something as complex and variable as behavior -- even the behavior of a fish with a brain the size of a pinhead.
1, 2 Widely distributed in Southeast Asia and northern Australia, this Gram-negative bacillus can cause a diversity of acute, sub-acute, and chronic clinical manifestations, which are frequently unrecognized because of the difficulties of accessible laboratory diagnosis.
C. gattii is an emerging pathogen that has triggered serious public health concerns due to (i) its appearance in previously unknown geographic areas, (ii) its outbreaks among healthy humans, pets, and wildlife, (iii) the intractable nature of cryptococcal disease, and (iv) the difficulty of diagnosis in clinical laboratories.
The tendency to avoid pre-matured tissues may come down to both the high cost of extended culture periods in a regulated laboratory, the difficulty of providing evidence of potency and purity when growing a complex tissue and the challenge of integrating pre-matured tissue with surrounding natural tissues at the implantation site.
