Due to the difficulty of amplifying the entire LRR gene (HM00024) in H. erato and other species in the pupal mating clade, analyses included only H. charithonia (a monomorphic species closely related to H. erato), three races of H. melpomene (malleti, cythera, plesseni), and BAC sequences of H. erato and H. numata.
The difficulties are amplified at schools that have a high student-to-faculty ratio, who lack a pool of graduate students and postdoctoral researchers who might serve as mentors, or are generally undercapitalized.
However, the hemolytic phenotypes of these atypical isolates suggest that despite the difficulty in amplifying this locus there were no gross alterations in Hly function.
In the case of Healthy Schools, this difficulty is amplified by the partnership approach embedded within the philosophy and by the possibility that schools who have not engaged with Healthy Schools programmes may be accessing other health promotion programmes and interventions.
The policy difficulties were amplified by an ideological cleavage within the House GOP.
In the presence of nonlinearities, these difficulties are amplified by the need of solving, at each step, some nonlinear algebraic systems whenever implicit schemes are adopted to cope with stability issues.
Among the limitations faced by in vivo AAV display library selection is the difficulty to rescue and amplify tissue-targeted library viruses for multiple selection rounds as the amplification systems used in vitro are based on adenoviral superinfection and can therefore not easily be applied in living animals.
The recent Treatment Options for Type 2 Diabetes in Adolescents and Youth trial amplifies the difficulty of treating type 2 diabetes in children and adolescents and further emphasizes the need to identify preventative approaches for this unique high-risk population (17).
Although the 0 3 cmbsf sediment samples were taken just below the bacterial mat, no sequences for Beggiatoa spp. were found, consistent with the frequently observed difficulty to amplify full-length Beggiatoa spp. 16S rRNA sequences from mixed environmental samples [47].
Due to difficulty in amplifying the entire length of kinesin from many species, analysis of kinesin was limited to exon 13 (1748 bps), the largest exon and also the region that includes the predicted "binding domain" of the protein.
These difficulties are amplified as its use expands, along with the transition from attributional (aLCA) to consequential (cLCA) and from retrospective to prospective.
