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To begin to define the nature of the emerging satellite cell defect in muscles of some mdx mice, we exposed satellite cells from 6 month old control or poorly-differentiating mdx animals to IGF-1 (100 ng/ml) either in the proliferation phase, the differentiation phase or both (P+IGF, D+IGF, P+IGF D+IGF, respectively; Fig. 5).
If the expansion phase is considered to result in a change in phenotype towards a proliferative nonprotein secreting cell, the differentiation phase is the opposite.
To confirm the BrdU+GS+opsin+ phenotype unambiguously and estimate the proportion of such cells, retinal explants at the end of the differentiation phase were dissociated and subjected to triple immunofluorescence analysis.
At the end of the differentiation phase, the SP compartment was found depleted of cells and GS+BrdU+ cells were now observed in the NSP compartment (Fig. 7E), which contains post-mitotic precursors and differentiated cells.
We observed delayed synthesis of milk proteins in the Elf5+/− gland where increased expression of Elf5 protein was delayed until 16.5dpc, suggesting that these mammary glands are not entering the differentiation phase of the developmental program until this time.
The separation of ErbB2 from ErbB3 induced by Muc4 is thought to trigger the 'switch' from the proliferative phase to the differentiation phase in mammary epithelial cells [29], [31].
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However, recently a direct Fra-2 transcriptional regulation of COLL I and OSTC genes in humans has been demonstrated, suggesting a possible involvement of both Fra-1 and Fra-2 in all the differentiation phases of OBs.
The present study demonstrates that low oxygen percentage applied during the differentiation phases of human articular chondrocyte culture enhances cell biosynthetic activity as well as reduces the activity of catabolic enzymes known to play key roles in the breakdown of cartilage matrix during degenerative pathologies.
Because the proposed protocol carries out one round of the collision resolution process in the priority differentiation phase, the optimal k is 6 for the contention resolution phase among helpers with the same priority.
During the terminal differentiation phase, the pre-adipocyte develops the characteristics of a mature adipocyte, including the accumulation of large lipid droplets and the conversion to a rounded cell shape (Rosen and MacDougald, 2006).
In the proposed protocol, the priority differentiation phase can terminate early as long as one or more helpers with a higher priority participate in the contention.
More suggestions(15)
the separation phase
the variation phase
the deviation phase
the comparison phase
the identification phase
the fragmentation phase
the gap phase
the division phase
the differentiation issue
the toddler phase
the approach phase
the differentiation level
the negotiation phase
the revolution phase
the differentiation strategy
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