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The differences in support between the different alignments were much larger than between likelihood and parsimony.
The differences in support between the different alignments were much larger than between the phylogenetic optimality criteria applied (maximum parsimony and maximum likelihood).
The figure also shows the considerable difference in accuracies between Rate4Site when run on these alignments and when run as part of ConSurf; this difference is likely a result of the different alignments used.
Although differences are apparent in the trees obtained using the different alignments, the fundamental conclusions made about the distinct clustering of cod, zebrafish and percomorph repeats are the same regardless of alignment parameters.
It has, however, been shown that known regulatory elements are not always correctly aligned (Siggia, 2005), an issue that is further complicated by the different alignments produced by various alignment programs (Pollard et al., 2004).
For example, on an erasable slick surface, a viewer with a black marking pen in hand can practice "oculama," the science of conveying expression through the different alignments of eyelids and pupils.
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Given the different alignment efficiencies of software, clean reads were aligned using TopHat2 [ 74] (v2.0.12) with Bowtie [ 75] (v0.12.9.0) for single-end reads (49 bp) and Bowtie2 [ 76] (v2.1.0.0) for paired-end reads (90 and 150 bp).
The different alignment results are achieved by using various electrode designs and SWNT solutions with various concentrations.
The schematic diagram in Figure 1c illustrates the different alignment, densification, and morphological distribution of PCDTBT nanotubes resulted between 2 and 24 h of immersion time.
We compared the effectiveness of the different alignment algorithms by using the likelihood values returned by maximum-likelihood phylogeny.
SAT requires that the user provide the input sequence data and select the different alignment parameters.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com