Herein we describe the development of a nested-PCR for the detection of B. henselae and B. clarridgeiae based on the strategy of species-specific size differences in the 16S-23S rDNA intergenic region that includes an Internal Amplification Control for PCR inhibitors.
The development of a novel nested polymerase chain reaction is described and used for detecting the presence of Neospora caninum and Hammondia heydorni DNA in DNA extracted from feral rodent tissues.
We describe the development of a two-step nested polymerase chain reaction (PCR) protocol to amplify the triosephosphate isomerase (TPI) gene of G. duodenalis and G. microti and nucleotide sequence characterization of amplified TPI fragment.
"I'm delighted that we see even a few atoms trapped," says Gabrielse, who pioneered studies of cold antiprotons and the development of the nested Penning trap.
Various factors suggested to play a role in the development of nested mutualistic networks [45], [46] probably also contribute to the nested structure documented here.
The aim of this paper is to present the development of an interventional cardiac nursing curriculum nested within a Master of Nursing Practice in Australia.
Examples include the development of nested multi-scale studies and experiments conducted over large spatio-temporal scales at the level of estuary or region.
Perhaps acknowledging the layers of complexity relating to health promotion theories, a public health approach, the domains of health literacy and a population perspective, will enable them to be neatly packed up like Russian nesting dolls, to facilitate the development of a suitable framework to assess the critical health literacy of communities.
The development and validation of a nested RT-PCR methodology for the detection of Mammaglobin A-mRNA-positive circulating tumor cells in peripheral blood of patients with operable breast cancer and evaluation of its prognostic significance.
These two equations fully determine the development of an annual primitively eusocial bee colony from nest founding at time step i = 0 until the end of the season (i = L).
