Exact(2)
For that purpose, both assays were used for the determination of viability and EC50 values.
The determination of viability of the trypsinized adherent cells by the trypan blue exclusion assay demonstrated only a slight and non-significant decrease in viability after 24 h hypoxic exposure (BHY: 97.1 %; FaDu: 98.7 %) compared to normoxic conditions (BHY: 99.1 %; FaDu: 99.4 %) (Table 1).
Similar(58)
The determination of cell viability was accomplished on cell viability analyzer (Vi-CELL XR, Beckman Coulter, Fullerton, CA, USA) using elimination test with trypanose blue.
Sensitive detection of cell necrosis is crucial for the determination of cell viability.
One sample of the particular type was used for the determination of the viability of the cells [9].
The determination of cell viability depends on these physical and biochemical properties of cells.
Conditions for the origin of partial sporophytic self-incompatibility (SSI) are obtained from two quantitative models, which differ with respect to the determination of offspring viability.
However, the natural strong fluorescence of QDs could interfere with signals during the determination of cell viability and create inaccuracies in the obtained data.
Afterwards, the cells were washed and the determination of cell viability was carried out after addition of WST-1 reagent and formazan measurement, as described in the manufacturer instructions manual.
The determination of cell viability was performed by MTT assay [ 16].
An alternative method for the determination of cell viability is fluorescent staining.
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