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However, in both experiments, the detection of unexpected familiar and novel stimuli was faster than the detection of expected familiar and novel stimuli.
We analysed mtDNA heteroplasmy in transfected H39 cells using Southern blotting, allowing the detection of expected mtDNA haplotypes, as well as potential, undesired recombined mtDNA species (Bacman et al, 2009) (Fig 3D).
This is consistent with power analysis simulations conducted by Magwene and Colleagues (2011) examining at the effects of sample size and sequencing coverage on the detection of expected QTL.
Consistent with the fMRI experiment, the detection of expected stimuli was accompanied by longer RTs than unexpected ones as indicated by the main effect of expectation (F1,36 = 28.87, P < 0.001; familiarexpected: M = 1,562 ms, SD = 229 ms; familiarunexpected: M = 1,434 ms, SD = 315 ms; novelexpected: M = 1,591 ms, SD = 261 ms; novelunexpected: M = 1,394 ms, SD = 228 ms).
PCR amplification employed on each individual DNA sample (T. evansi and B. bigemina) using their specific primers led to the detection of expected fragments of size 257 and 689 bp, respectively.
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Therefore the BacR assay should allow the detection of the expected high levels of ruminant pollution and the prediction of E. coli levels in spring water.
As a positive control for the detection of the expected deglutamylation product, Cys-Gly-3M3SH was included as an additional sample (sample 7).
Specific comments for consideration in a revised submission: 1) The conclusion that the mRNAs are endonucleolytically cleaved is based on the detection of the expected 5′ mRNA fragment in a Ski2∆ strain (which blocks 3′ to 5′ degradation).
Specific response to Reviewers' comments: 1) The conclusion that the mRNAs are endonucleolytically cleaved is based on the detection of the expected 5′ mRNA fragment in a Ski2∆ strain (which blocks 3′ to 5′ degradation).
For the detection of the expected product 3-methyl-crotonyl-CoA, UPLC-MS analysis was performed on samples containing ATP, CoA, β-valine, BvaA-His, and either BvaB1-His or BvaB2-His.
In addition, these regions displayed increased effective connectivity during the detection of unexpected versus expected stimuli, with important memory formation regions within the MTL; the hippocampus (connecting with both SN/VTA and GP) and the perirhinal cortex (connecting with GP).
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