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The described method is based on Poisson processes.
The described method increases the effectiveness of the NVD design.
The described method only assumes that two common constraints are met.
The described method was experimentally tested to avoid bias from the magnification of the images.
Extraction of RNA and cDNA was carried out using the described method.
We present some examples to comprehend overview and demonstrate the efficiency of the described method.
A testing implementation of the described method was made available (Additional file 6).
The described method enables accurate quantitative partial volume correction in oncological hot spot imaging.
For the verification of the described method, experiments are presented in a standard Fizeau interferometer.
The described method can be used only when the data is sufficiently available.
Filling ratio is analytically obtained using effective medium layer in section B of the described method.
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