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Presence of adapter sequences in the datasets was checked with cutadapt utility (Martin, 2011).
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The dataset was checked for duplicate entries.
The dataset was checked three times for discrepancies.
The dataset was checked for non-Mendelian inheritance errors using the prepare option in CRI-MAP.
The dataset was checked for completeness and consistency and compared with the results of any publications.
The dataset was checked by the senior statistician and translated into a final dataset following data cleaning procedures.
The degree of agreement between the allocated NHS number and the NHS number supplied in the dataset was checked and used to calculate specificity and sensitivity values.
The normality of the distribution of datasets was checked with Kolmogorov-Smirnov test.
To avoid this problem, we adopted a stringent filtering criteria where each protein of these datasets were checked to ascertain that their interaction was either physically validated by some other experiment or they had a direct functional implication in the disease pathogenesis as reported in the literature.
The data quality of log2-transformed RPKM values for these two RNA-Seq datasets was checked using the sample histogram.
The data quality of log2-transformed RPKM values for these 7 RNA-Seq datasets was checked using parallel plot and heat-map dendrogram.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com