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Cross-population comparisons of maternal and paternal lineages based on the frequency of haplogroups or rates of continental ancestry common to all samples in the database were performed using ARLEQUIN 3.11 [ 17].
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Data processing and insertion into the database was performed using multiple purpose-tailored scripts in python and R (R Development Core Team, 2010).
Analysis of all the microarray data incorporated in the database was performed using LIMMA (24), a Bioconductor (25) package for the R statistical programming language (http://www.r-project.org).org
The linkage procedure among the databases was performed using anonymous code, created for each patient by the Local Administrative Authority, which is the health provider legally authorized for the processing of sensitive data.
For the examples shown here, the comparison between the reference tree and the databases was performed using the browser or the data sets.
Two online searches of the National Library of Medicine MEDLINE database were performed using the PubMed search engine.
Comparisons with sequences available in the NCBI/GenBank database were performed using the BLAST [ 2] and CDD [ 20] resources.
Profile searches against the NR database were performed using the PSI-BLAST (Altschul et al., 1997) and the HMMSEARCH and JACKHMMER programs of the recently developed HMMER3 package (Eddy, 2008).
Comparisons of DNA sequences and their deduced amino acid sequences with the EMBL/GenBank database were performed using the BLAST programs blastn, blastx, and blastp [23].
PSI-BLAST searches on the NCBI protein database were performed using different representatives of the YRG family as seed, according to the bibliography, and were iterated until members of the closest subfamily were found in the list of hits.
BLAST searches against the NCBI NR protein database were performed using Blast2GO version 2.5.0, using blastx with an e-value cut-off of 1e-5.
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