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The cultural method revealed diverse but limited number of bacteria.
Either the decolonization regimen lead to suppression of the ESBL-producing bacteria in the intestinal tract below the detection limit of the cultural method used rather than to true eradication (and renewed selection pressure due to reapplication of antimicrobial therapy in often highly comorbid patients drives 'outgrowth' of ESBL-E).
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We also cover the cultural methods that offer scope to support synergies between the aforementioned methodological combinations.
Another drawback is that the cultural methods are time consuming which might delay possible control measures.
Additionally, identification and susceptibility testing of the isolates are major advantages of the cultural methods except that they take relatively long growth time.
In conclusion, the cultural methods in the present study were shown to be surprisingly equivalent in terms of accuracy, sensitivity and specificity for different feed materials and serotypes, respectively.
The cultural methods compared in this study are primarily developed to detect Salmonella in different food samples and since the level of intrinsic flora is usually high in animal feed [ 14] the isolation of Salmonella from feed might be affected.
The objective of this study was the development of DNA and RNA real-time PCR methods for detection of food-borne Salmonella sp. as rapid alternatives to the traditional cultural method (ISO 6579, 2004) in fresh meat carcasses and processed meat samples.
The results of this study showed that the accuracy, sensitivity and specificity of the investigated cultural methods were equivalent.
The purposes of this study were to develop a new cultural method for the rat bone marrow stromal cells (MSCs) to differentiate into osteoblasts well in vitro, and to investigate the feasibility of using MSCs as seed cells and three-dimensional porous nano-hydroxylapatite as scaffolds for constructing tissue-engineered bone.
The standard cultural methods are primarily developed for food materials and only very few feed materials have been included in the validations studies.
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