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The number of CTB-immunoreactive neurons in the mPFC was lower when the CTB immunohistochemistry was conducted in sections previously subjected to in situ hybridization (data not shown).
Here we constructed CTB-Fim2 to explore Fim2 immunogenicity exploiting the adjuvant properties of the CTB molecule.
As news of the CTB sale filtered through, Brewdog removed all CTB beers from its bars.
At the announcement of the CTB buyout, its founder, Jasper Cuppaidge, talked of making it "world famous".
Wendy Yen, the CTB official who oversaw the research department, has since left the company to work for the Educational Testing Service, which administers the SAT.
He announced that, for the first time, school officials would be judged by how well their students did on the CTB tests.
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For quantifying VGLUT1 immunoreactivity, the dLGN ipsilateral to the CTB-injected eye was photographed and examined as follows.
The CTB-labeled rafts were then crosslinked with anti-CTB antibody in order to create distinct raft patches on the plasma membrane [77] and visualized by confocal microscopy.
It should be noted that some VGLUT2-immunoreactive puncta near the optic tract were located outside of the CTB-positive area (Figure S3, arrowheads).
We found that the extent of the VGLUT2-positive area largely coincided with that of the CTB-positive area (Figure S3C, D).
Approximately half (∼46%) of the CTB-labeled cells in the pan-ventronasal domain were GFP-positive after MTN injection (Figure 5E, G; Table 1; 279/603).
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